Water_Analysis[1]

Water_Analysis[1] - Water Quality Analysis Part I and II...

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Water Quality Analysis Part I and II
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Introduction : The objective of the lab experiment was to test the quality of water through various analyzation methods. Water was collected from the same site of the Kansas River on two different occasions and the same tests were conducted to each sample. A few tests were done on site, including temperature readings and preliminary pH tests, but most were done at the lab session. The tests done measured the phosphate concentration of the water, the pH, the nitrate concentration, the total water hardness, the turbidity, the presence and quantity of fecal coliform, and the quantity of total dissolved solids. The phosphate was found by creating five known concentrations of a phosphate solution, diluting the phosphate by increasing the ratio of water to phosphate, collecting the absorption of each sample, then plotting the absorbance vs. concentration using excel and finding an equation to the line. That information was then used along with the absorbance of our water sample to find its phosphate concentration. The pH was found using a pH probe. The nitrate concentration was found by using the provided contraption that sucked water sample into it where the water mixed with certain chemicals. The color then had to be matched with a wheel that told you the nitrate concentration. The total water hardness was found by titrating EDTA into a solution of sample water, a buffer, an indicator, and ascorbic acid until the endpoint was reached. The turbidity was found using turbidimeter. Fecal coliform was found by putting growth medium in a Petri dish, applying it to the given filter, filtering the sample water with vacuum assistance, sealing the dish and placing it in an oven for 45 minutes, flipping it and checking it 48 hours later. The total dissolved solids were found using a conductivity probe. This is a real world type of experiment and addresses the advantages of being able to use analytical chemistry to your advantage. It displays the importance of having the tools we’ve talked about and worked on all year mastered and at your disposal. The
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scientific background is that many bodies of water are not as pure as you would think; they have various pollutants and being able to detect those pollutants is imperative to limiting their extent. The expected results were mostly unknown because there can be significant variation in the contents of different bodies of water. Experimental : Phosphate - The first part of the two part lab started by the acquiring of the 1.00 x 10 -1 M stalk solution already prepared. Next a stock of 1.00 x 10 -3 M phosphate solution was prepared. From the student prepared 1.00 x 10 -3 M phosphate solution, five standard solutions with phosphate concentrations of 2.00 x 10 -5 , 5.00 x 10 -5 , 1.00 x 10 -4 , 2.00 x 10 -4 , and 5.00 x 10 -4 M were prepared and placed into a flask. Then 5.00 mL from each flask was pipetted into five separate beakers and 1.00 mL of ammonium molybdate and 0.400 mL of aminonaptholsulfonic acid was
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Water_Analysis[1] - Water Quality Analysis Part I and II...

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