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Midterm%202WhiteKey

Midterm%202WhiteKey - Name White Key Last First BIS102-02...

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Name White Key BIS102-02, Spring '08, Page 1 of 9 Last, First C. S. Gasser Student Authorization: I, , authorize the University to distribute publicly this graded exam ( e. g. , handed out in class, or left in a bin for me to pick up). Signature Date BioSci 102-02 May 20, 2008, Second Midterm Instructions: There are nine pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. For calculation problems a clear line of calculations leading to your final answer must be shown to obtain full credit. Use the following pK a values for the exam. Amino Acid α -NH 2 α -COOH R-group log 1.0 = 0.00 Ala, Gly, Ile, Leu, Val 9.7 2.3 log 1.5 = 0.18 Asn, Gln 9.0 2.1 log 2.0 = 0.30 Met, Ser, Trp 9.3 2.3 log 2.5 = 0.40 Phe 9.1 1.8 log 3.0 = 0.48 Pro 10.6 2.0 log 3.5 = 0.54 Thr 10.4 2.6 log 4.0 = 0.60 Asp 9.8 2.1 3.9 log 4.5 = 0.65 Glu 9.7 2.2 4.2 log 5.0 = 0.70 His 9.2 1.8 6.0 log 5.5 = 0.74 Cys 10.8 1.8 8.3 log 6.0 = 0.78 Tyr, Lys 9.1 2.2 10.0 log 6.5 = 0.81 Arg 9.0 2.2 12.5 log 7.0 = 0.85 log 7.5 = 0.88 Question Value Score log 8.0 = 0.90 1 8 log 8.5 = 0.93 2 21 log 9.0 = 0.95 3 15 log 9.5 = 0.98 4 12 log 10.0 = 1.00 5 12 6 16 R = 8.3 X 10 -3 kJ/°K mol 7 9 25°C = 298K 8 14 9 12 10 10 11 9 12 12 Total 150
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Name White Key BIS102-02, Spring '08, Page 2 of 9 Last, First C. S. Gasser 1. The figure below represents an isoelectric focusing (IEF) gel on which three protein samples (A, B, and C) were run to completion. The locations of the two electrodes are indicated by the “+” and "-" signs. a) (4) Which sample includes a protein with the highest pI? (circle one) A B C High pI is a basic protein that will initially have a “+” charge and will move toward the “-“ pole. The illustration below shows the result of sodium dodecyl sulfate (SDS, and also including ß-mercaptoethanol) polyacrylamide gel electrophoresis of the same samples that were examined on the IEF gel above. The gel was stained with commassie blue dye. The direction of migration was from top to bottom. A B C M b) (4) The size markers used on the gel are in the lane labeled “M” and are 10 kilodaltons (kD), 20 kD, 50 kD, and 90 kD in molecular weight. What is the approximate molecular weight of the protein visible in sample “B”? 50 kD, smaller proteins move more rapidly through the gel so the markers go from smallest to largest going from bottom to top of the gel. The band in B migrates with the 50 kD marker. + - A B C
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Name White Key BIS102-02, Spring '08, Page 3 of 9 Last, First C. S. Gasser 2. In the space to the left of each of descriptions below, write the letters corresponding to all of the methods to which that statement applies. All correct answers must be given for full credit. (3 points each) A. Gel filtration.
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