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Unformatted text preview: b) Biochemical experiment: Isolate plasma membrane fractions from both normal and CF cells. Run plasma membrane proteins out on an SDS-PAGE Transblot gel and probe blot with a labeled antibody specific for CFTR protein Compare lane with normal membrane proteins with lane with CF sample Lets suppose that the above hypothesis was proven to be correct. Describe what experiment you would do next to further our understanding of the role of CFTR protein in this disease. c) Experiment: Create a DNA construct containing sequence coding for normal CFTR protein linked to a reporter gene, such as GFP; link this to strong promoter. Transfect CF cells with this construct. Confirm expression of CFTR in PM of host cells and determine if this restores chloride efflux function. (gain of function)...
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- Summer '08