2008WinterFinalKey

2008WinterFinalKey - BIS101-001/Chan Winter 2008, Final...

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BIS101-001/Chan Winter 2008, Final exam version A Key 1) Using the list of words on the next page, write on the line the term best described by the following expressions (30 pts): A structural change in chromosome such that: AB.CDEFGHIJ is an ___ Inversion ____ AB.CDHGFEIJ Bacterial artificial chromosomes that overlap to span a genomic region____ Clone contig ____ The failure of homologous chromosomes to separate properly in meiosis results in nondisjunction during this meiotic division ____ Meiosis I ________ DNA sequence that can copy itself and move to new genomic locations __ Transposon_ ___ Chromosome rearrangement that can create gene families during evolution ____ Duplication ____ Method used in genomic research that relies on the ability of double stranded RNA to post- transcriptionally inactivate genes __ RNAi _ Recessive mutations in these genes contribute to cancer _____ Tumor suppressors _____ Process that corrects wrongly-incorporated bases after DNA replication __ Mismatch repair__ Making a mutant from a gene to infer function ___ Reverse Genetics ___ A mutation that confers a new property to the protein is a _ Gain-of-function A cell or individual that has three copies of a given chromosome instead of two ___ Trisomic ___ A cis acting sequence that activates eukaryotic gene expression __ Enhancer __ Cellular process triggered by premature stop codons in mRNA__ Nonsense-mediated decay The process of making RNA from DNA _ Transcription _ Type of genetic experiment in many organisms are grown, then tested individually for a given phenotype __ Screen_
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Transcription Forward genetics Null mutation Replication Transcription factor RNAi Transposon Nonsense-mediated decay Reverse genetics Translocation Tumor suppressors Oncogenes Trisomic Meiosis I Base excision repair Duplication Inversion Crossing-over Monosomic Nonsense mutation Gain-of-function Clone contig Screen PCR Meiosis II Mismatch repair Selection Conditional Silencer VNTR Shotgun sequencing Enhancer Deletion Note: You may only use a word once, but there are more words than definitions. You may remove this sheet if it will make it easier for you to fill in the blanks on the previous page. You may also use this as scratch paper.
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2) You have identified a new species of bacteria that has the unusual property of being resistant to ionizing radiation. To identify the genes controlling this trait, you mutagenize the bacteria with EMS and screen for mutants that are sensitive to ionizing radiation. Fortunately, you are able to transform the bacterial species at high efficiency. In order to construct a genomic library, you digest the wild-type genomic DNA and vector with EcoRI, ligate them together, transform into E.coli and select on plates containing kanamycin. a)
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This note was uploaded on 08/18/2008 for the course BIS 101 taught by Professor Simonchan during the Winter '08 term at UC Davis.

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2008WinterFinalKey - BIS101-001/Chan Winter 2008, Final...

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