Midterm2BKey

Midterm2BKey - BIS101-001 Simon Chan Winter 2008 Midterm 2...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
BIS101-001 Simon Chan Winter 2008 Midterm 2 Version B Key 1. (14 pts) Below is the structure of gene Z. Assume that the +1 of the transcription start site is at the junction of 1 and 2, and that translation begins in the middle of 4 and ends in 10. For parts b through e, use the number provided at the top of each section to describe that section. 1 2 3 4 5 6 7 8 9 10 11 Promoter Exon Intron Exon Intron Intron Exon Exon Intron Exon a. Is this gene from a eukaryote or a prokaryote? Explain your answer in the simplest possible terms (4 pts) This is most likely a eukaryotic gene because it has introns. b. What parts may not be present in the mature transcript of gene Z if there is alternate splicing? (2) 4,7,8,10 c. Draw a diagram of RNA derived from 6 after mRNA processing is complete. Indicate base pairs that are almost always found in introns (4) U G AG A (A is at branch point with 2’ OH linkage to 5’ phosphate of G) d. Where would TFIID likely bind? (2) 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
e. Will gene Z be functional if there are the following mutations? Yes or No answers (2) Mutation Functional Z? (yes, no, maybe) i. 50 base pair deletion in the middle of part 5 Yes ii. 2 base pair deletion in part 6 Maybe (for this question, assume that translation ends in 10) Note: for 6e-ii the deletion may have affected GU or AG bases required for splicing. 2. (10pts) A friend has asked you to characterize the genomes of three recently isolated viruses, named Davirus1, Davirus2, and Davirus3. She gives you a sample of each genome at a concentration of 10 μ g/ ml. You perform a denaturation experiment and see the following: a) (3 pts) Indicate if the viral genomes are single stranded or double stranded: Davirus1 single stranded Davirus2 double stranded Davirus3 double stranded b) (2pts) What does the denaturation kinetics tells you about the Davirus2 and Davirus3 genomes? Davirus3 has a higher GC content than Davirus2. c) (3pts) Analysis of base composition reveals the following Davirus1: 30% guanine; 30% adenine; 20% cytosine; 20% uracil Davirus2: 20% guanine; 30% adenine; 20% cytosine; 30% thymine Davirus3: 30% guanine; 20% adenine; 30% cytosine; 20% thymine
Background image of page 2
Indicate the type of nucleic acid each viral genome is made of. Davirus1: RNA Davirus2: DNA Davirus3: DNA d) (2pts) Renaturation kinetics of Davirus2 and Davirus3 show the following: Which virus has a larger genome? Davirus2 must have a larger genome because it took longer to reanneal suggesting fewer molecules in the 10 μ g. 3.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 08/18/2008 for the course BIS 101 taught by Professor Simonchan during the Winter '08 term at UC Davis.

Page1 / 8

Midterm2BKey - BIS101-001 Simon Chan Winter 2008 Midterm 2...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online