bis_104_pq_5_ans_ss_i_08 - did he arrive at based on the...

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BIS 104 PQ 5 SS I 08 5. To further investigate the possible role of peripheral nerves in mouse limb skin arteriogenesis, Mukouyama made use of the Neurogenin 1 / Neurogenein 2 (Ngn1 / Ngn2) double homozygous mutant strain of mice. It had been established previously that these mice lack peripheral sensory nerves. Describe the experiments that Mukouyama did with these mice. Limb skin samples from both normal and mutant E15.5 mice were immunostained with fluor. abs. against PECAM-1; ephrinB2, BFABP, 2H3, an CX-40. The staining patterns from the two sample sources were compared by scanning confocal fluor. microscopy. What conclusion
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Unformatted text preview: did he arrive at based on the results of these experiments? PSNs are required for the differentiation of small arterial blood vessels in the mouse limb skin. In addition to the differentiation markers that you have encountered in experiments described in figs 1 – 3, figure 4 describes the expression of two additional markers; BFABP (brain fatty acid binding protein) and alpha-SMA (alpha-smooth muscle actin). What cell types are specifically identified by these two markers? BFABP is expressed only by Schwann cells. Alpha-SMA is expressed only by smooth muscle cells (seen only around developing arteries, not veins)....
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