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Unformatted text preview: . d) refractive index: Refers to the degree to which a substance (air, glass, liquid) causes diffraction of light waves. Denoted as “n”, it is a unitless value. E.g., refractive index of air = 1.0. e) numerical aperture (NA): A property of an objective lens. Defined as the product of the refractive index of the medium between the specimen and the obj. lens and sin alpha (the light-gathering ability of the lens). f) empty magnification: Refers to magnification beyond the resolving power of a microscope. It results in an increase in size of the image, but no increase or improvement in resolution or clarity of detail....
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- Summer '08
- refractive index, objective lens, objective lens mag