bis_104_pq_3_ans_ss_i_08

bis_104_pq_3_ans_ss_i_08 - microscopy. b. Describe the key...

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BIS 104 PQ 3 SS I 08 In their initial set of experiments (the results depicted in Figure 1 A – N, page 694) Mukouyama’s group obtained results that led them to conclude that: “Peripheral nerves associate preferentially with arterial vessels but not with veins.” These experiments were done using 15.5 day old (E15.5) mouse embryo limb skin. a. Briefly describe the design of the EXPERIMENT that led them to this conclusion. List the procedural steps in chronological order. EXPERIMENT: 1. Samples of E15.5 limb skin were taken from two strains of transgeneic mice: ephrinB2/lacZ and EphB4/lacZ 2. Both samples were stained for peripheral sensory nerves (2H3 marker) and for B-gal (EC artery or vein marker). 3. Stained samples were examined and compared by scanning confocal fluorescence
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Unformatted text preview: microscopy. b. Describe the key RESULTS of this experiment. The staining patterns for arterial markers co-localized with the branching pattern of the peripheral sensory nerves; the pattern of vein differentiation markers did not follow the nerve pattern. c. Do these results establish plausibility, necessity, or sufficiency? Explain the basis for your choice. They establish that it is plausible that the branching pattern and differentiation of peripheral sensory nerves and limb skin arteries influence each others development. There is a correlation in space between the two structures, but no proof of cause and effect....
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This note was uploaded on 08/18/2008 for the course BIS 104 taught by Professor Scholey during the Summer '08 term at UC Davis.

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