04-02-08 - 4-2-08TRANSCRIPTION-types of RNA mRNA, tRNA,...

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Unformatted text preview: 4-2-08TRANSCRIPTION-types of RNA mRNA, tRNA, rRNAmRNA-defined by pulse labeling as being unstable and short lived Sidney Brenner (concept of mRNA)-intermediate between DNA and protein-concept of intermediate led to concept of transcriptase, repressors & inducers Jacob Monodoproduct can either up-regulate or down-regulate the synthesis through feedback (cycle)rRNA (Ribosomal RNA)-assay TCA precipitation (small to large)-precursor is amino acid -product is protein-has to be mRNA dependent and so you need a whole ribosomal synthesizing system including tRNA, ATP/GDP, amino acids, etc.-this is a cell-free protein synthesizing systemO O O OO-O-O-Oamino acids protein-you can find an mRNA, even though it is unstable by using an f2 phage (RNA phage that is stable codes for 3 proteins){Zinder f1 & f2 phages}Cell-Free System-want to induce genetic change and want it to happen in 20 minutes-use f2 to program the cell-free system-as a control instead of using f2 used polyU (UPUPUPUP)opolynucleotide phosphorylase invented by Marianne Grunberg-Manga...
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04-02-08 - 4-2-08TRANSCRIPTION-types of RNA mRNA, tRNA,...

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