CHAPTER 9 (Figures)

CHAPTER 9 (Figures) - David L. Nelson and Michael M. Cox...

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Lehninger Principles of Biochemistry Fourth Edition Chapter 9: DNA-Based Information Technologies David L. Nelson and Michael M. Cox
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DNA Cloning Cutting DNA at precise locations Selecting a small molecules of DNA capable of self-replicating ( cloning vectors ) Joining the two DNA fragments (vector + target DNA) – Recombinant DNAs Moving recombinant DNA from test tube to a host cell. [Transfection or Transformation] Selecting or identifying host cells that contain recombinant DNA.
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Illustration of DNA cloning
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Cloning Vector
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Bacteriophage cloning vector
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Bacterial artificial chromosome (BACs) as cloning vector
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Construction of yeast artificial chromosome
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Use of hybridization to identify a clone with a particular DNA segment
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Probe to detect the gene for a protein of know amino acid sequence.
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DNA sequences in a typical E. coli expression vector.
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Two approaches to site-directed mutagenesis.
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This note was uploaded on 08/21/2008 for the course BIOCHEM 301 taught by Professor Vanes during the Spring '08 term at Rutgers.

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CHAPTER 9 (Figures) - David L. Nelson and Michael M. Cox...

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