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CHAPTER 9 (Figures) - David L Nelson and Michael M Cox...

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Lehninger Principles of Biochemistry Fourth Edition Chapter 9: DNA-Based Information Technologies Copyright  ©  2004 by W. H. Freeman & Company David L. Nelson and Michael M. Cox
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DNA Cloning Cutting DNA at precise locations Selecting a small molecules of DNA capable of self-replicating ( cloning vectors ) Joining the two DNA fragments (vector + target DNA) – Recombinant DNAs Moving recombinant DNA from test tube to a host cell. [Transfection or Transformation] Selecting or identifying host cells that contain recombinant DNA.
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Illustration of DNA cloning
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Cloning Vector
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Bacteriophage cloning vector
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Bacterial artificial chromosome (BACs) as cloning vector
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Construction of yeast artificial chromosome
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Use of hybridization to identify a clone with a particular DNA segment
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Probe to detect the gene for a protein of know amino acid sequence.
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