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Unformatted text preview: Technical Abstract Experiment #4 Determining Chlorophyll-A Concentrations in Plants and Algae: An Introduction to Spectrophotometry Introduction A good indicator of the heath of plants is the concentration of chlorophyll, which is a molecule that absorbs sunlight in photosynthesis, the foundation of plant survival. Spectrophotometry is a analytical technique that can be used to measure chlorophyll concentrations. The goal of this experiment is to prepare a calibration curve using the chlorophyll standard to determine the concentration of chlorophyll-a in a spinach leaf through the extraction of the chlorophyll. Materials and Methods A spectrophotometer was used to determine the wavelength of maximum absorbance for chlorophyll- a. Then serial dilutions of a stock solution of chlorophyll were used to prepare a calibration curve. Chlorophyll was then extracted from a spinach leaf, by grinding and filtering with acetone and the spectrophotometer was used to determine the absorbance of the extracted sample, which was then...
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