Lab 7-12 Review Catherine

Lab 7-12 Review Catherine - Lab 7: Growth cycle of...

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Lab 7: Growth cycle of bacteriophage •Plaque : Infected E.coli or free parental phage. •E. coli overnight: used to create a lawn of bacteria on the plate so plaques can be visible.
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Growth cycle of bacteriophage Latent period (20min.): time it takes the virus to infect the bacteria and reproduce plaques infected bacteria or free parental phages Rise period (progeny phase)(20min.): release of the progeny plaques progeny phages Length of growth cycle: mid-point of “rise period” ≈30minutes Burst size = # of plaques at 40min./ # of plaques at 10min.
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Effect of radiation on bacteria Exposed Serratia marcescens to UV light (different exposure time) UV rays cause death or mutation in cells 0 sec: normal growth 5 sec: reduced # of bacteria 10 and 15 sec: very few to none bacteria Exposure Curve??:
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Feulgen rx: specific for DNA resulting in the staining of the chromosomes a deep violet.
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Lab 7-12 Review Catherine - Lab 7: Growth cycle of...

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