Jean Sebastien Review

Jean Sebastien Review - Review of Lab material...

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Review of Lab material Jean-Sebastien Roy
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Disclaimer After the review session, you still have to study!!! Everything is not covered here. It just reminds you what we have seen…
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Lab 1 : microscope Parts and use of a microscope Magnification Staining (use and problems)
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Lab 1
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Lab 2 : Bacteria
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Lab 2 Morphology of bacterial cells and bacterial colonies
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Lab 2 Gram Staining (Why? How(steps)? p.2-7) Reagents: Basic dye (gentian violet) Mordant (iodine) Decolorizing agent (alcohol) Counter stain (carbol fuchsin) What’s the purpose of the mordant? Fix the dye to the bacteria Why did we add the counter stain? To visualize gram negative bacteria (pink) Manipulation Sterile procedures Inoculating loop Spreader Test tubes Petri plate
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Lab 2 Bacterial Growth How can we determine it? (procedure) Growth phase of a culture Generation time : B b n = 2 n : number of cell generations b : number of cells at 120 minutes B : number of cells at zero time
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Lab 3 : Cellular physiology Movement of molecules Diffusion
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Lab 3 Osmosis
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Lab 3 Active Transport
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Lab 3 : experiments Permeability of cell membrane 3 different size of acohols Bigger acohol = more fat soluble 3 different concentrations Higher concentration gets inside faster Results : (how to determine penetration?) Bigger enters more then others (enters at low concentrations) Membranes behaves like …
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Lab 3 : experiments Transport of chloride into turnip cells
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Jean Sebastien Review - Review of Lab material...

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