HW4 key - BMEN 289 Engineering Biology Homework Set #2 Due...

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BMEN 289 Engineering Biology Homework Set #2 Due Tuesday, Sept. 28 1. The rate equations from the handout (Equations 2-4) still apply, but now Equation 2 is set to zero: dC S /dt = -k 1 C E C S + k -1 C ES = 0 Now K D* = k -1 / k 1 = C E C S / C ES Solving for C E and inserting into the balance on the enzyme gives, So, * * 0 0 * K K + + K D ES D S E E ES ES ES S S E S ES D S C C C C C C C C C C C C C + = = = = + From Equation 8, 0 max 2 2 * * E S S ES D S D S dP C C V C V k C k dt K C K C = = = = + + so K M = K D*
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Hyperbolic curve 0 0.01 0 0.02 0.04 0.06 0.08 0.1 [S] V exp real 2. Here are a set of initial reaction rates vs. initial substrate concentrations. Plot the data and answer the questions below. Column 1: Initial concentration of substrate before starting the reaction Column 2: Actual initial reaction velocity Column 3: Experimentally-measured reaction velocity (with experimental error) Column 4: Experimentally-measured reaction velocity in the presence of an inhibitor S[mM] V real V measured V inhibitor 0 0 0 0 0.003 0.0042 0.005 0.0031 0.007
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This note was uploaded on 08/30/2008 for the course BMEN 289 taught by Professor Kaunas during the Spring '07 term at Texas A&M.

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HW4 key - BMEN 289 Engineering Biology Homework Set #2 Due...

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