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lecture 5 - I II III IV DNA Fingerprinting in a nutshell...

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I. DNA Fingerprinting in a nutshell II. Individual differences in DNA VNTRs III. RFLP analysis and dna fingerprinting IV. Difficulties and controversies Why are we interested in dna fingerprinting? - real fingerprints not left behind in many crimes - they are essentially unique dna fingerprinting what are the uses? - solve unsolvable crimes - resolve paternity issues - can prove innocence of people unjustly convicted - immigration dna is a long sequence of “letters” that carry the info of how to build molecules for the body adenine, thymine, guanine or cytosine how much “spelling” difference is there between individuals? - almost none - 99.9% identical - different enough; 3 million different base pairs take home msg 1: on average, individuals differ in their dna sequence at 0.1% of their bases: 3 million differences out of 3 billion base pairs the fundamental question - we have dna from several samples how can we tell if the dna came from the same person?
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