Lecture 9 ATP Synthase Energetics

Lecture 9 ATP Synthase Energetics - BME 418 Quantitative...

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BME 418, Quantitative Cell Biology Alan J. Hunt 9 - energetics of ATP-synthase ATP synthase structural points: - F 0 stoichiometry and arrangement determination. - What happens of b subunit cut? - Consists of two opposed reversible motors. Characteristics of ATP synthase (draw membrane and #s) : - G ATP = 67 kJ/mol = ~25 k b T/molecule = ~100 pN nm/molecule. - Reversible - Proton concentrations (pH). ~8.4 in matrix, 7.0 in intermembrane space - Size of structures ~ 20 nm for whole structure. How much energy is available per proton? - start with energy due to the concentration difference (from Boltzmann): 1 2 2 1 ln c c RT U U = - this works out to 8.31*300*ln(10e-7.0/10e-8.4) = 8.0 kJ/mol - however, there is also a charge separation, membrane behaves as a capacitor: C = Q/E 1.0 μ F/cm 2 for a typical cell membrane - potential across inner membrane = 0.15 V (how were V and pH determined?) - How much energy is released when one mole of protons is moved across a 0.15 V potential? Since
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This note was uploaded on 09/06/2008 for the course BIOMEDE 418 taught by Professor Hunt during the Winter '08 term at University of Michigan.

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Lecture 9 ATP Synthase Energetics - BME 418 Quantitative...

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