Discussion_2008_03_14 - Tissue culture Mutant/drug #1 t...

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Tissue culture Δt Δt Mutant/drug #1 Mutant/drug #2
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Cell cycle Time Cell 3 Cell 2 Cell 1 Blue=interphase Red=mitosis
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Cell cycle Time Cell 3 Cell 2 Cell 1 Remove drug/condition Blue=interphase Red=mitosis
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What controls the cell cycle? Yeast mutants Look for mutations that appear to affect the cell cycle Some affect machinery Some affect regulation Hartwell (Nobel prize, 2001)
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Finding related regulatory molecules Look for mutations that are relatively likely to be related to one process/pathway Synthetic lethality Rescue Normal Mutation A Phenotype Mutation 2 Phenotype Cells dead Mutation 1 Phenotype
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Conditional mutations Mutation only has an effect under certain conditions TS: Temperature sensitive CS: Chemical sensitive Useful for determining sequence of events Restrictive conditions: conditions under which the mutation has an effect i.e., if not under restrictive conditions, the mutation is still present, but it has no effect
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This note was uploaded on 09/06/2008 for the course BIOMEDE 418 taught by Professor Hunt during the Winter '08 term at University of Michigan.

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Discussion_2008_03_14 - Tissue culture Mutant/drug #1 t...

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