Discussion_2008_03_14 - Tissue culture Mutant/drug#1 t...

Info icon This preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Tissue culture Δt Δt Mutant/drug #1 Mutant/drug #2
Image of page 2
Cell cycle Time Cell 3 Cell 2 Cell 1 Blue=interphase Red=mitosis
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Cell cycle Time Cell 3 Cell 2 Cell 1 Remove drug/condition Blue=interphase Red=mitosis
Image of page 4
What controls the cell cycle? Yeast mutants Look for mutations that appear to affect the cell cycle Some affect machinery Some affect regulation Hartwell (Nobel prize, 2001)
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Finding related regulatory molecules Look for mutations that are relatively likely to be related to one process/pathway Synthetic lethality Rescue Mutations A & B Normal Mutation A Phenotype Mutation 2 Phenotype Mutations 1 & 2 Cells dead Mutation 1 Phenotype
Image of page 6
Conditional mutations Mutation only has an effect under certain conditions TS: Temperature sensitive CS: Chemical sensitive Useful for determining sequence of events Restrictive conditions: conditions under which the mutation has an effect i.e., if not under restrictive conditions, the mutation is still present, but it has no effect
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern