ProblemSet8

ProblemSet8 - BICD100 Genetics Fall 2005 Reinagel PROBLEM...

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BICD100 Genetics Fall 2005 Reinagel PROBLEM SET 8 FORWARD GENETICS (Lecture Nov 8) 1) In bacteriaphage λ you are studying three mutations that cause plaques to be either speckled, blue, or diffuse. You cross a speckled, blue, diffuse strain to wild type λ by co-infection (all bacteria are infected with both phages). You plate the resulting lysate and analyze the phenotypes of the plaques caused by the progeny phage: Phenotype Number of Plaques Speckled, blue, diffuse 2121 Speckled, blue 3 Speckled, diffuse 344 Blue, diffuse 21 Speckled 22 Blue 349 Diffuse 4 Wild type 2136 Derive the most accurate map you can of the order of these genes and the distances between them, indicating any ambiguities.
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BICD100 Genetics Fall 2005 Reinagel 2) You isolate a new bacterial species E. oilspillcleani that is able to break down crude oil into carbon dioxide and water. Tests show that your wild type strain can break down decane, eicosane, naphthalene, phenanthrene, and pyrene, and it is naturally tolerant to high temperature conditions (temp R ). You isolate a temperature sensitive variant of the wild type parent, and separately derive two different Hfr strains from it: dec+ eic+ nap+ phe+ pyr+ temp S Hfr 1 dec+ eic+ nap+ phe+ pyr+ temp S Hfr 2 Starting from the wild type again, you also obtain a strain that has mutations in 5 genes required for hydrocarbon degradation: dec1 eic1 nap1 phe1 pyr1 temp
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ProblemSet8 - BICD100 Genetics Fall 2005 Reinagel PROBLEM...

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