PS10_Key2v2

PS10_Key2v2 - BICD100 Genetics Fall 2005 Reinagel Problem...

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BICD100 Genetics Fall 2005 Reinagel Problem Set 10 = Practice Questions for Final Exam This is not a practice final (it does not have the same number of questions as a real final etc.) It is just a set of additional practice problems to help you get ready for the final. For additional practice see Chapter 16: Problems 1, 5, 17, 39. 1) (this is Chapter 6 problem 4) Several mutants are isolated, each of which requires compound G for growth. The compounds (A to E) in the biosynthetic pathway to G are known but their order in the pathway is not known. Each compound is tested for its ability to support growth of each mutant (1 to 5). In the following table, a plus sign indicates growth and a minus sign indicates no growth: Compound tested A B C D E G Mutant 1 - - - + - + 2 - + - + - + 3 - - - - - + 4 - + + + - + 5 + + + + - + a. what is the order of compounds A to E in the pathway? EABDG b. At which point in the pathway is each mutant blocked? 1 B --> D 4 A -->B 2 A --> B 5 E -->A 3 D --> G c. Would a heterokaryon composed of double mutants 1,3 and 2,4 grow on a minimal medium? 1,3 and 3,4? 1,2 and 2,4 and 1,4? Yes NO Yes -+-++ -+-++ --+++ +-+-+ ++--+ +-+-+ -++-+
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BICD100 Genetics Fall 2005 Reinagel 2) (this is Chapter 6 problem 20) A yeast geneticist irradiates [mutagenizes] haploid cells of a strain that is an adenine- requiring auxotrophic mutant, caused by a mutation in the gene ade1 . Millions of the irradiated cells are plated on a minimal medium, and a small number of cells divide and produce prototrophic colonies. These colonies are crossed individually to a wild- type strain. [Note: this means a haploid mutant x haploid wild type are mated to create a diploid, and then the diploid undergoes meiosis and the progeny haploid
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PS10_Key2v2 - BICD100 Genetics Fall 2005 Reinagel Problem...

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