bio hw6 answers

bio hw6 answers - 1D.) Chip 2 11 9 6 7 8 10 12 Chip 1 5 1 2...

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1D.) 2 a.) One method for making many copies of a fragment of DNA is called Polymerase Chain Reaction, which involves cloning a piece of DNA in a test tube rather than in living cells like E. coli. The steps involved are strand separation, primer annealing, and strand synthesis. The process can be repeated for multiple cycles, each resulting in a twofold amplification of DNA. The second method is called cDNA derived by reverse transcription. This method involves creating cDNA from mRNA, and the result is DNA representing the transcript without any introns. b.) The advantages of creating cDNA is that it is devoid of introns, and much smaller than the genomic DNA for a gene, making it easier to study. Also, it is tissue-specific, meaning you obtain different results depending on where you obtain it from. On the other hand, PCR allows massive amplification of DNA in a very short time, and it selectively duplicates a particular DNA fragment based on the choice of primers used, and you don’t have to put it in cells. This method has
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bio hw6 answers - 1D.) Chip 2 11 9 6 7 8 10 12 Chip 1 5 1 2...

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