BIOL%20438%20Lecture%202_082908

BIOL%20438%20Lecture%202_082908 - Bacterial Transcription...

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Bacterial Transcription DNA DNA RNA Protein Functional Protein Replication Transcription Translation Modification Reverse Transcription During the biomolecular reactions of replication , transcription , and translation information is passed from one class of molecule to the next. This flow is not always completely unidirectional, i.e., RNA can be reversibly transcribed into DNA in higher organisms or during retrovirus replication. The Flow of Genetic Information in Cells Source: R. Wagner, Transcription Regulation in Prokaryotes (2000) The Central Dogma
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Nucleic Acid Structure The nucleic acids, DNA and RNA are polymers of nucleotides linked together by phosphodiester bonds DNA and RNA differ in the nitrogenous bases they contain the sugars they contain whether they are single or double stranded
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Figure 11.5
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Nucleic Acid Structure Deoxyribonucleic Acid (DNA) Polymer of nucleotides Contains the bases adenine, guanine, cytosine and thymine Sugar is deoxyribose Molecule is usually double stranded
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Nucleic Acid Structure Ribonucleic Acid (RNA) Polymer of nucleotides Contains the bases adenine, guanine, cytosine and uracil Sugar is ribose Most RNA molecules are single stranded
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Transcription RNA synthesis under the direction of DNA RNA produced has complementary sequence to the template DNA Three types of RNA are produced mRNA carries the message for protein synthesis tRNA carries amino acids during protein synthesis rRNA molecules are components of ribosomes
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Bacterial Transcription The ‘players’ or cellular components necessary for transcription Transcription start sites: the core promoter A site on DNA to which RNA polymerase binds to initiate transcription
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RNA polymerase (RNAP) The machinery that catalyzes DNA-dependent RNA synthesis is the enzyme RNA polymerase . Subunit composition of bacterial RNA polymerases Core enzyme [E] Holoenzyme [Eσ] 2 × α 2 × α 1 × β 1 × β 1 × β′ 1 × β′ 1 × ω 1 × ω 1 × σ Core enzyme alone can catalyze the synthesis of RNA but lacks specificity. The role of sigma is in recognition of the
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This note was uploaded on 09/01/2008 for the course BIOL 438 taught by Professor Thompson during the Fall '08 term at Purdue University-West Lafayette.

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BIOL%20438%20Lecture%202_082908 - Bacterial Transcription...

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