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Microbiology Lab - Experiment 3-8 Capsular Staining(pgs...

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1 Experiment 3-8: Capsular Staining (pgs. 95-96) Purpose : To learn the procedure for doing the capsular stain Materials and Methods: Milk cultures of : Stain: Klebsiella pneumoniae Congo Red Staphylococcus epidermidis Maneval’s Stain Procedure: 1. Perform negative stain with Congo Red 2. Air dry the slide 3. Flood slide with Maneval’s stain for 1 minute 4. Rinse with water 5. Blot dry with Bibulous paper 6. Examine under oil-immersion Capsular staining is a combination of two staining techniques : Negative stain : using Congo Red to color the background Simple stain : using Maneval’s stain to color the cell proper of the bacteria The capsule does not take up stain and appears colorless between the dark background and the stained bacteria. K. pneumoniae Background stained with Congo Red Bacteria proper stained with Maneval’s stain Capsule remains colorless Capsules : - polysaccharide layer is generally referred to as a glycocalyx
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