enzymelab

enzymelab - Jennifer OBrien Bio Lab 2/8/06 Introduction Lab...

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Jennifer O’Brien Bio Lab 2/8/06 Introduction Lab Title: Enzyme Kinetics: Factors Affecting Activity In this lab, the main focus is looking at the effects of four different  variables on the activity on an enzyme.  For some background information,  enzymes are proteins.  More specifically they are specialized catalysts and that  speed up a chemical reaction without undergoing any chemical changes  themselves.  Each enzyme has a substrate.  A substrate is the ligand that the  enzyme bonds to and converts them into chemically modified products.  Each  enzyme is very specific, in that it only catalyzes one reaction.  Also, each  enzyme has an active site, the special bonding area on its surface, which  supports the substrate.  In our experiment, we are dealing with the enzyme B- galactosidase.  The substrate is o-nitrophenyl-B-D-galactoside (ONPG).  The  substrate or products of this catalytic reaction are galactose and a yellow-
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enzymelab - Jennifer OBrien Bio Lab 2/8/06 Introduction Lab...

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