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Unformatted text preview: b. For comparison, compute k B T as an order-of-magnitude estimate of the thermal energy available at room temperature (25 °C). Give the answer in eV. B (Boltzmann’s constant k B = 1.38 x 10 J/K) B-23 5. a. Given below, the Henderson- Hasselbalch equation describes the relative amounts of protonated and unprotonated species that will exist in a solution at a certain pH. pH = pK +log([A-]/[HA]) Protonation and deprotonation of amino acid side chains is important for the function of many proteins. Using the values in the chart below (Biochemistry by Stryer, 2002), calculate the percentage of terminal carboxylic acid groups that would be unprotonated at pH 1, 2, 3, 4, 5, 6, 7, 8, 9, 10. Do the same for terminal amino groups and plot that percentage as a function of pH. (This is much less tedious if you use Excel or another spreadsheet program.) b. Draw the form of a lysine amino acid that would predominate at cytosolic pH = 7....
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- Spring '05
- ELSER, V