Lab_Report_6_-_Absorption_Spectroscopy

Lab_Report_6_-_Absorption_Spectroscopy - Absorption...

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Absorption Spectroscopy Abstract: A sample of lemon-lime Kool-Aid was tested using absorption spectroscopy to determine the amount of yellow #5 and blue #1 dyes present. Using a serial dilution, four standard solutions for each dye were made from stock solutions with known concentrations. The standards were used to create calibration plots to compare the results of the Kool-Aid measurements and help perform calculations. From these calculations, yellow #5 was determined to have a molar absorptivity of 2.56x10 3 M -1 and composed .249% of the sample. Blue #1 was determined to have a molar absorptivity of 1.38x10 4 M -1 and composed .0357% of the sample. Introduction The main objective of this experiment was to determine the amount of dye present in a sample of Kool-Aid using absorption spectroscopy. The secondary objectives were to “collect absorbance measurements from a simple spectrophotometer, perform a serial… dilution to prepare standards, perform a Beer’s law analysis to determine a solution’s
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Lab_Report_6_-_Absorption_Spectroscopy - Absorption...

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