Mid%202%20Winter%202008

Mid%202%20Winter%202008 - 1 of 2 BIS 102 Name Winter 2008...

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Unformatted text preview: 1 of 2 BIS 102 Name__________________________________________________ Winter, 2008 Last First K. Hilt Second Midterm Score (100): ___________ Equations: pH = pK a + log {[b]/[a]} K a = x 2 /(y-x) K b = x 2 / (y-x) pH = (pK a 1 + pK a 2 )/2 (K a )(K b ) = 1 x 10-14 F = (q 1 q 2 ) / ε r 2 ∆ G = ∆ H - T ∆ S Amino acid pK a ’s: α-carboxyl group (2.1), α-amino group (9.6) side chains: D (3.9) E (4.2) H (6.0) C (8.3) Y (10.1) K (10.5) R (12.5) Oligopeptide pK a ’s: C-terminal carboxyl group (3.6), N-terminal amino group (7.4) 1. ( 10 pts.) A protein was completely digested with arg-C and an internal peptide (i.e. not derived from the N- or C-terminals) was purified. Determine its sequence from the following information: a) amino acid analysis of one mole of the peptide yields three moles C, one mole M, three moles K, one mole R, and one mole W; b) treatment of the intact peptide with trypsin gives a dipeptide containing R and W, a dipeptide containing K and C; and a tripeptide containing C, M, and K; c) treatment of the intact peptide with Edman’s reagent, followed by acid...
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This note was uploaded on 09/11/2008 for the course BIS 102 taught by Professor Hilt during the Summer '08 term at UC Davis.

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Mid%202%20Winter%202008 - 1 of 2 BIS 102 Name Winter 2008...

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