deletion mapping handout key

deletion mapping handout key - 1 A set of six rII deletion...

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1. A set of six rII deletion mutants were crossed with each other in all possible combinations with the following results (+ = recombinants produces, i.e., able to grow on E.coli strain K; 0= no recombinants). 1 2 3 4 5 6 1 0 + 0 + + + 2 0 0 + + 0 3 0 0 + 0 4 0 0 0 5 0 + 6 0 Draw a deletion map consistent with these data. To answer this question, first you want to draw a line to indicate the overall rII locus: Then you want to start analyzing the pairs of crosses to see which pairs CANNOT produce wild-type growth (where there is a “0” or “-“ on the table). 1 can complement every other mutation except for 3, 2 can complement every other mutation except for 3 and 6, 3 only complements with 5, 4 is only complemented with 1 and 2, 5 can complement every other mutation except for 4, and 6 is only complemented by 1 and 5. Since mutant 3 seems to encompass the largest deletion (since there is only one other mutation that can complement it), I like to start by drawing that mutant as a relatively
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deletion mapping handout key - 1 A set of six rII deletion...

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