deletion mapping handout

deletion mapping handout - recombinants were obtained....

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1. A set of six rII deletion mutants were crossed with each other in all possible combinations with the following results (+ = recombinants produces, i.e., able to grow on E.coli strain K; 0= no recombinants). 1 2 3 4 5 6 1 0 + 0 + + + 2 0 0 + + 0 3 0 0 + 0 4 0 0 0 5 0 + 6 0 Draw a deletion map consistent with these data. 2. Five deletion mutants for the erg-B gene were isolated, and when crossed to each other gave the following: (+ = recombinants, - = no recombinants) 1 2 3 4 5 1 - + - + - 2 - - - - 3 - + - 4 - - 5 - a) Draw a deletion map of the erg-B gene using this matrix. b) There were ten point mutants of the erg-B gene available: they were crossed with each of the 5 deletion mutants, and the crosses were scored for the appearance of erg-B+ recombinants. The data follow, in which a plus again indicates that erg-B+
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Unformatted text preview: recombinants were obtained. Point Mutants Deletion Mutants i ii iii iv v vi vii viii ix x 1 - + + - + - - + - - 2 + - - + - + + - + + 3 - - - - + - - + - - 4 + + + + - + + - + + 5 - - - - - - - - - - Assign each of these point mutants to one of the areas defined by your deletion map. c) A complementation analysis was performed using the same ten erg-B alleles. The following results were obtained, in which a plus indicates strong complementation and a minus, no complementation at all. i ii iii iv v vi vii viii ix x i - + + - + - - + - + ii + - - + - + + - + - The rest of this matrix is unnecessary. From this information alone: 1) how many cistrons are there in the erg-B region? And 2) Which alleles are in which cistron?...
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