mak119bul - Activity Assay Kit Catalog Number MAK119...

This preview shows page 1 - 2 out of 2 pages.

Acetylcholinesterase Activity Assay Kit Catalog Number MAK119 Storage at Room Temperature TECHNICAL BULLETIN Product Description Acetylcholinesterases (AChEs) are enzymes that hydrolyze the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE is located at the synaptic cleft and functions to terminate synaptic transmission by catalyzing the breakdown of ACh allowing cholinergic neurons to return to a resting state after activation. Changes in AChE activity may result from exposure to certain insecticides, which act as cholinesterase inhibitors. Inhibitors of AChE are also used to treat certain conditions such as dementia. The Acetylcholinesterase Activity Assay kit provides a simple and direct procedure for measuring AChE levels in a variety of samples such as blood, serum, and plasma. This assay is an optimized version of the Ellman method in which thiocholine, produced by AChE, reacts with 5,5 -dithiobis(2-nitrobenzoic acid) to form an colorimetric (412 nm) product, proportional to the AChE activity present. One unit of AChE is the amount of enzyme that catalyzes the production of 1.0 μ mole of thiocholine per minute at pH 7.5 at room temperature. This kit has a linear range of 10–600 units/L of AChE activity.
Image of page 1

Subscribe to view the full document.

Image of page 2
  • Spring '17
  • Andriani
  • room temperature, ache activity, Acetylcholinesterase Activity Assay, activity assay kit

{[ snackBarMessage ]}

Get FREE access by uploading your study materials

Upload your study materials now and get free access to over 25 million documents.

Upload now for FREE access Or pay now for instant access
Christopher Reinemann
"Before using Course Hero my grade was at 78%. By the end of the semester my grade was at 90%. I could not have done it without all the class material I found."
— Christopher R., University of Rhode Island '15, Course Hero Intern

Ask a question for free

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern