Worksheet 7_Replic_Spr17

Worksheet 7_Replic_Spr17 - Worksheet 7 DNA structure and...

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Worksheet 7: DNA structure and DNA replication - Refer Chapter 16 Refer minilecture on Quest LM 5 Learning Objectives: 1) Describe and interpret experiments that demonstrated DNA is the genetic material of cells and viruses – Griffith, Avery, McCleod and McCarty, Hershey and Chase. 2) Predict the orientation and sequence of complementary strand based on nucleotide sequence 3) Describe the three proposed models of DNA replication. 4) Describe and interpret results from Messelson-Stahl experiment that showed DNA replication was semiconservative. 5) What is ORI sequence? 6) Name and describe the functions of enzymes/proteins in DNA replication 7) What are some unique traits of DNA polymerases – need a primer, can make DNA only in one direction. 8) Explain how replication differs in leading and lagging strands 9) Be able to label a replication bubble with ori, replication forks, leading, lagging strands etc 10)Be able to draw leading strands, Okazaki fragments in a given replication bubble and label all 5’ and 3’ ends.
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