Lab Final Review - Exercise 1 The proper way to handle and...

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Exercise 1 The proper way to handle and use a microscope Relationship between magnification and field of vision The oil immersion lens and its proper use When oil should be used on a microscope slide oil immersion lens Know how to calculate the total magnification ocular x objective Exercise 2 Difference between Brownian movement and true motility Brow- not true movement but rather movement caused by the molecules in the liquid shaking, True- keeps water still so that you can see the organism move The use of wet-mount technique and how it is done a fast method of observing microbes, shake infusion-transfer loopful-place coverslip and press down The use of hanging-drop procedure and how it is done to view motility and larger organisms, obtain depression slide-toothpick with petroleum jelly-touch edges of coverslip-place drop of infusion on coverslip-place depression slide face down on coverslip-invert slide with coverslip so drop is hanging Exercise 3 Why agar is used in media (its function) few microbes can degrade agar so it remains solid during microbial growth, it can remain solid up to 100 C The difference between nutrient broth and nutrient agar broth is liquid complex medium, agar is the solid medium that results when agar is added to nutrient broth How autoclaving works, how it sterilizes uses steam under pressure, 121 C at 15 psi for 15 min Know the meanings of the following terms: Inoculate intentionally introduced of microbe Turbid cloudy liquid media
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Colony population of cells that arises from a single bacterial cell Pellicle membrane on surface of broth Know the colony descriptions in Figure 3.3 (pg 28) circular, irregular, biconvex, filamentous, rhizoid. EDGES: entire, undulate, lobate, filamentous, curled Exercise 4 Know the differences between broth cultures, agar slants, and agar deeps When/Why they’re used broth culture: provide large number of bacteria in a small easily transported space. Slants: agar solidified at an angle, provide solid growth surface but easier to store than petri plates. Deeps: used to grow bacteria that require less oxygen, have less agar so can show when bacterium is motile How/why the bactincinerator is used sterilizes loops/needles with infrared heat Be able to demonstrate the following using the proper procedures and tools: Nutrient broth inoculation Agar slant inoculation
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