Exp. Immuno (Short Version) - Experimental Immunology Techniques You Should Know or learn on your own DNA Sequencing Polymerase Chain Reaction DNA

Exp. Immuno (Short Version) - Experimental Immunology...

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Experimental Immunology
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Techniques You Should Know or learn on your own - DNA Sequencing - Polymerase Chain Reaction - DNA Footprinting/Nucelase protection - Restriction enzyme manipulation - Nucelase protection - cDNA Synthesis - Site-Directed Mutagenesis
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What Does It Mean: “To Clone”? Clone: a collection of molecules or cells, all identical to an original molecule or cell To "clone a gene" is to make many copies of it - for example, by replicating it in a culture of bacteria. Cloned gene can be a normal copy of a gene (= “wild type”). Cloned gene can be an altered version of a gene (= “mutant”). Recombinant DNA technology makes manipulating genes possible.
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Restriction Enzymes Bacteria have learned to "restrict" the possibility of attack from foreign DNA by means of "restriction enzymes”. Cut up “foreign” DNA that invades the cell. Type II and III restriction enzymes cleave DNA chains at selected sites. Enzymes may recognize 4, 6 or more bases in selecting sites for cleavage. An enzyme that recognizes a 6-base sequence is called a "six-base cutter”.
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Basics of type II Restriction Enzymes No ATP requirement.
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