8Sep08 - Lecture overview Microbial nutritional...

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Lecture overview • Microbial nutritional requirements and  classification • Prokaryotic transport systems • Measuring microbial growth 1
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Microbial nutritional requirements • Basic requirements are sources of carbon precursors  (12-13); source of electrons that can be energized,   energy source • Movement of high energy electrons leads to  production of ATP and a proton motive force (PMF) • Microbes vary in basic ways in which they obtain  these nutritional requirements and this is used in  classification • Sources of nitrogen, sulfur, phosphorous, iron and  some other minerals also required in order to make  all cellular components from precursors 2
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Classification of microbes based on sources of  nutritional requirements 3
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Prokaryotic transport systems • Uptake of nutrients requires special  transporter because CM is impermeable  (except to small gas molecules) • Facilitated diffusion is not major  mechanism for prokaryotic transport • Active transport moves solutes into cell  against a concentration gradient, requires 
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8Sep08 - Lecture overview Microbial nutritional...

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