Transformation in the Bacteria of E coli

Transformation in the Bacteria of E coli - Transformation...

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Transformation in the Bacteria of E coli Paresh Jha 2/11/08 Section 6
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For many years the scientific community knew that the transformation of cells was possible thanks to Frederick Griffith way back in 1928. The only problem was discovering what substance made transformation possible. In 1944, Oswald Avery made that exact discovery. He essentially used the process of elimination by destroying likely candidates that would carry the transformational properties. If he destroyed the right chemical then transformation would be halted and that material would subsequently have the transforming property. Avery discovered that DNA was that hereditary material. The Transformation in the Bacteria of E coli experiment clearly demonstrates the transformation process and deepens an understanding of Avery’s monumental discovery. The purpose of this laboratory exercise is to simply transform an E coli cell into an E coli cell with an ampicillin resistance gene. The resistance gene gives the E coli the ability to resist damage from the ampicillin antibiotic when it is growing on a plate with ampicillin. This lab consists of four major components and therefore we have specific expectations (hypothesis) for each part. The first portion of our lab involves the E coli cells we are using. Our first expectation is that if the β-mercaptoethanol is added properly then the cell membrane will become more permeable. The second portion of the lab involves our DNA with a plasmid holding the ampicillin resistance gene. Our hope here is that DNA fuses properly with E coli . Then next critical part of the experiment has to do with incubating the transformed cells
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This note was uploaded on 09/16/2008 for the course MCB 213 taught by Professor Zhang during the Spring '08 term at UConn.

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Transformation in the Bacteria of E coli - Transformation...

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