Lec_5 - Chapter 3 II. PROTEIN FUNCTION All Proteins Bind to...

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Chapter 3 II. PROTEIN FUNCTION
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All Proteins Bind to Other Molecules Figure 3-36 Molecular Biology of the Cell (© Garland Science 2008) Selective binding via precise docking - complementary surfaces. Driving forces - multiple weak interactions.
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Protein surface determines its function Amino acid side chains at surface and their relative orientations are key
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Figure 3-39 Molecular Biology of the Cell (© Garland Science 2008) Evolutionary trace method helps identify binding sites and underscores how amino acid sequence identity between proteins yields similar structures and function.
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measure binding strength - equilibrium constant for the binding reaction: A + B <=> AB
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A couple of hydrogen bonds can drastically affect binding. ∆G = -RTlogK Decreasing ∆G by 1.4 kcal/mole raises K by a factor of 10 at 37 o C.
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Enzymes Are Powerful, Highly Specific Catalysts -ase Catalysts (not used up) Permit cells to make or break bonds in controlled way Enzymes speed up reaction rates by stabilizing transition state
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Description of enzyme catalysis: E + S -> ES -> EP -> E + P Figure 3-46 Molecular Biology of the Cell (© Garland Science 2008) Transition for ES to EP goes through a transition state intermediate that is stabilized by the enzyme.
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Lec_5 - Chapter 3 II. PROTEIN FUNCTION All Proteins Bind to...

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