An Observation of Transformation in E. coli

An Observation of Transformation in E. coli - An...

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An Observation of Transformation in E. coli By Edward Acie Trimble
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Table of Contents Title .......................................................................................................................................... Pg. 1 Table of Contents ..................................................................................................................... Pg. 2 Abstract .................................................................................................................................... Pg. 3 Introduction ............................................................................................................................. Pg. 4 Methods ................................................................................................................................ Pg. 5-7 Results .................................................................................................................................... Pg. 8 Conclusion ............................................................................................................................... Pg. 9 Citations ................................................................................................................................. Pg. 10
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Abstract In this project, we wanted to see how transformation functions and its effect on the bacteria E. coli . To observe this, we developed a procedure in which we placed E. coli into 4 different environments: a Luria agar plate with 10 uL of pUC8 plasmid, a Luria agar plate without a plasmid, a Luria agar plate with ampicillin and 10 uL of pUC8 plasmid, and a Luria agar plate with only ampicillin. We repeated this procedure again except we changed the agar plates with 10 uL of pUC8 plasmid to have 15 uL of plasmid instead. The results showed that the E. coli with 15 uL of pUC8 plasmid were the only ones to grow bacteria colonies (7 colonies).
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Introduction In order to develop different cellular functions, the field of biotechnology has developed methods to cause changes in DNA. Under natural circumstances, transformation, involves the release of plasmid DNA from donor cells into the surrounding medium; subsequently recipient cells are able to take up the DNA from the medium, ( ). Plasmids are circular molecules that contain DNA and are located in most bacteria. E. coli, a bacteria, only takes up this DNA only under certain conditions. Biotechnology has developed the treatments of electroporation and calcium chloride heat shock. In the heat shock procedure, DNA is dissolved in a solution of divalent cations; the positive ions associate with the negatively charged DNA
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