8-29 - Bacteria (Eubacteria) Archaea (Archeabacteria)...

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Unformatted text preview: Bacteria (Eubacteria) Archaea (Archeabacteria) Eukarya Informational processes Metabolism Techniques in Cell and Molecular Biology Microscopy The light microscopy Phase-contrast microscopy Fluorescence microscopy Confocal scanning light microscopy Transmission and scanning electron microscopy Freeze-refraction replication (Chapter 18, p733-780) Pulse-chase experiment Differential centrifugation Polyacrylamide gel electrophoresis X-ray crystallography Immunolocalization The Light Microscopy The Light Microscopy Empty Magnification Resolution d = 0.61 n sin Numerical Aperture (N.A.): n sin Aberrations optical flaws n is the refractive index of the medium. The optical quality of an objective lens is measured by its resolution. Diffraction The resolution obtained by a microscope is limited by diffraction. Visibility and Contrast Refractive index: n A part of a specimen can be seen because it affects the light differently from its adjacent parts and its background Oil Phase-Contrast Microscope...
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8-29 - Bacteria (Eubacteria) Archaea (Archeabacteria)...

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