8-29 - Informational processes Bacteria(Eubacteria...

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Bacteria (Eubacteria) Archaea (Archeabacteria) Eukarya Informational processes Metabolism
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Techniques in Cell and Molecular Biology Microscopy      The light microscopy Phase-contrast microscopy Fluorescence microscopy Confocal scanning light microscopy      Transmission and scanning electron microscopy Freeze-refraction replication (Chapter 18, p733-780) Pulse-chase experiment Differential centrifugation Polyacrylamide gel electrophoresis X-ray crystallography Immunolocalization
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The Light Microscopy
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The Light Microscopy
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Empty Magnification
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Resolution d =  0.61  λ n sin  α Numerical Aperture (N.A.):  n sin  α Aberrations – optical flaws n is the refractive index of the medium. The optical quality of an objective lens is measured by its    resolution. 
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Diffraction The resolution obtained by a microscope is limited by diffraction.
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Visibility and Contrast Refractive index: n  A part of a specimen can be seen because it affects the light differently from its adjacent parts and its background  Oil
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