4th_Exam_Solutions_2007

4th_Exam_Solutions_2007 - Question 1 You have just cloned...

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Question 1 You have just cloned the gene for a new growth hormone receptor. You have expressed and purified the protein and determined that in the presence of the hormone the receptor’s kinase activity is stimulated. You want to learn more about the molecular architecture of your receptor and performed a ligand-binding experiment. Below is the graph and 3 data points from the graph: Kinase activity as function of free ligand 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0 1 2 3 4 5 [X] relative kinase activity [X] rel. kinase act. 1.0 0.02 2.0 0.5 3.0 0.9 a) Use the three data points to determine the Hill coefficient for this binding curve. Answer: The Hill coefficient is 5.55.
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b) The number you got for the Hill coefficient will probably not be an integer. What is the relationship between the Hill coefficient and the likely number of binding sites on the receptor? Answer: The Hill coefficient gives the number of binding sites in the case of absolutely perfect cooperativity. Such perfect cooperativity rarely exists in any real molecule. Therefore the number of ligands that are required to bind
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4th_Exam_Solutions_2007 - Question 1 You have just cloned...

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