lab2bioabstract - B Subtilius and added to SB100...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Genetic mapping of critical proteins in tryptophan, tyrosine, and histodine pathways for Bacillus Subtilis , the SB100 strain Bacterial transformation is a well documented phenomenon which allows for exchange of genetic material between cells and their environment, this relationship can be used to determine the recombination frequency for genes and thus a chromosomal position. B. Subtilius undergoes these transformations, and contains critical metabolic pathways that are prone to knockout in prepared strains. Here I studied the uptake of genetic material and frequency of recombination to establish a genetic map for 3 genes, encoding critical proteins in the pathways for tryptophan, histidine or tyrosine. Wild type DNA was isolated, extracted and purified from wild type
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: B. Subtilius and added to SB100 auxotroph (a trp- tyr- his- strain) allowing for transformation. The transformed B. Subtilius were then plated on selective media, lacking between 0 and all 3 of the required amino acids; colonies that grew were then once again replated on different selective culture media. Of the total 144 colonies grown, 22 were (his+tyr-), 2 were (tyr+his-), 8 were (tyr+his-), 19 were (trp+tyr-), 3 were (trp+his-), and 16 were (his+trp-). I found the order of the genes was trp-his-tyr with tyr-his map distance of 16%, trp-tyr map distance of 21.5% and his-trp map distance of 13%. Future experiments will increase the number of total bacterial platings, generating more accurate resulting by having more total colonies to analyze results....
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern