lab2bioabstract - B. Subtilius and added to SB100 auxotroph...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Genetic mapping of critical proteins in tryptophan, tyrosine, and histodine pathways for Bacillus Subtilis , the SB100 strain Bacterial transformation is a well documented phenomenon which allows for exchange of genetic material between cells and their environment, this relationship can be used to determine the recombination frequency for genes and thus a chromosomal position. B. Subtilius undergoes these transformations, and contains critical metabolic pathways that are prone to knockout in prepared strains. Here I studied the uptake of genetic material and frequency of recombination to establish a genetic map for 3 genes, encoding critical proteins in the pathways for tryptophan, histidine or tyrosine. Wild type DNA was isolated, extracted and purified from wild type
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: B. Subtilius and added to SB100 auxotroph (a trp- tyr- his- strain) allowing for transformation. The transformed B. Subtilius were then plated on selective media, lacking between 0 and all 3 of the required amino acids; colonies that grew were then once again replated on different selective culture media. Of the total 144 colonies grown, 22 were (his+tyr-), 2 were (tyr+his-), 8 were (tyr+his-), 19 were (trp+tyr-), 3 were (trp+his-), and 16 were (his+trp-). I found the order of the genes was trp-his-tyr with tyr-his map distance of 16%, trp-tyr map distance of 21.5% and his-trp map distance of 13%. Future experiments will increase the number of total bacterial platings, generating more accurate resulting by having more total colonies to analyze results....
View Full Document

This note was uploaded on 09/21/2008 for the course BIOL 18a taught by Professor Kosinski-collins during the Spring '08 term at Brandeis.

Ask a homework question - tutors are online