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bio207f08lec06 - Biology 207 Lecture six Monday 15 Sep 2008...

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Biology 207 Lecture six Monday, 15 Sep 2008 Bacterial physiology and metabolism – 1 microbial nutrition and culture energy and redox Chemical Composition of the Bacterial Cell: Bacterial cells  --  water, 80% of mass, inorganic ions (K, Na, Ca, Mg, Fe, etc.), small organic molecules (amino acids, sugars, fatty acids), (high concentration)  and large  molecules (polymers) (protein, nucleic acid, peptidoglycan (especially for bacterial cells), lipid, polysaccharide). Macronutrients :  C (50), O (20), N (14), H (8) P (3), S (1), Na (1), K (1), Ca (0.5), Mg (0.5), Fe (0.2)    (% of dry weight of cell) Protein, lipid, DNA, RNA, polysaccharides, peptidoglycan:  made up of C, O, H, N, S, P K -- enzyme activity (variety) Ca -- heat resistance of endospores, stability of the CW (outside of cell membrane holding things in, would be loose without calcium) Mg -- cofactor for enzymes, stability of membranes Fe -- part of cytochromes, cofactor for enzymes (electron transfer) Take this all in See Table 5.1 for sources of nutrients. (where macronutrients come from) co2 good source of carbon for auto trophs but mose we are talking bout are heterotrophs h2O, O2 get a feel for the list but don't MEMORIZE  it! Micronutrients (trace elements) needed as cofactors and parts of enzymes (0.2) (Cu, Zn, Mo, Bo, Se, Cl, Ni, Cr, Co, Wo) Mn, phosphatases; oxygen-detoxifying enzymes Zn, structure of enzymes, DNA and RNA polymerases Co, Vit B 12 (cofactor of viatmin B12) Cu, certain enzymes of respiration, oxygen-detoxifying enzymes Mo, enzymes of nitrogen metabolism (especially nitrogen fixing enzymes) Ni , hydrogenases Wo-toungston Some bacteria require one or more growth factors:   an organic compound that is essential for growth of a cell but that the cell cannot synthesize. Bacterica can  make almost everything but require certain components pre-formed  Must acquire factor intact from the environment, usually required in small amounts, different for different  bacteria. a) vitamins -- required in small amounts for growth and activity of the cell, but do not serve as carbon or energy sources.  (organic compounds the cell does not catabolize) b) amino acids, for protein synthesis.  c) purines and pyrmidines, for nucleic acid synthesis DIRECTED READING STUDY ASSIGNMENT used the book to find the difference between: Prototroph and phototroph Auxotroph
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