PCB Lab 1 - Name Eric Hunter Exercise 1 Cell Transport Mechanisms and Permeability Activity 1 Simulating Dialysis(Simple Diffusion Lab Report Pre-lab

PCB Lab 1 - Name Eric Hunter Exercise 1 Cell Transport...

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Name: Eric Hunter Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report Pre-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. The driving force for diffusion is You correctly answered: b. the kinetic energy of the molecules in motion. 2. In diffusion, molecules move You correctly answered: a. from high concentration to low concentration. 3. Which of the following dialysis membranes has the largest pore size? You correctly answered: d. 200 MWCO 4. Avogadro's number is a constant for the number of Your answer : a. moles. Correct answer: b. molecules. 06/10/17 page 1
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06/10/17 page 2 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No, not at all. Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : b. both glucose and albumin Stop & Think Questions: The reason sodium chloride didn't diffuse left to right is that You correctly answered: c. the membrane pore size was too small. Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because You correctly answered: d. glucose and albumin are both too large to pass. The rate of diffusion for urea You correctly answered: b. is slower than that for sodium because urea is a larger molecule. Experiment Data: Solute MWCO Left Solute Concentration Right Solute Concentration Average Diffusion Rate Na+ Cl- 20 9.00 0.00 0.0000 Urea 20 0.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na+ Cl- 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na+ Cl- 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na+ Cl- 50 9.00 0.00 0.0150 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na+ Cl- 50 18.00 0.00 0.0273 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na+ Cl- 100 9.00 0.00 0.0150 Urea 100 0.00 0.00 0.0000
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06/10/17 page 3 Solute MWCO Left Solute Concentration Right Solute Concentration Average Diffusion Rate Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na+ Cl- 100 0.00 0.00 0.0000 Urea 100 9.00 0.00 0.0094 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na+ Cl- 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 0.00 0.00 0.0000 Glucose 200 9.00 0.00 0.0042 Na+ Cl- 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 9.00 0.00 0.0000 Glucose 200 0.00 0.00 0.0000
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06/10/17 page 4 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. The effect of increasing the concentration of sodium chloride from 9 m M to 18 m M in the left beaker was to You correctly answered: b. increase the rate of diffusion. 2. Describe the difference between the rate of diffusion seen for sodium and urea.
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