Transpiration Lab - Natalie Cook AP Biology Title Transpiration Ms Denney 5 November 2007 Objectives To explore how water moves from roots to leaves in

Transpiration Lab - Natalie Cook AP Biology Title...

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Natalie Cook Ms. Denney AP Biology 5 November 2007 Title : Transpiration Objectives : To explore how water moves from roots to leaves in terms of the physical/chemical properties of water and the forces provided by differences in water potential, the role of transpiration in the transport of water within a plant, and the structures used by plants to transport water and regulate water movement. Hypothesis : I hypothesize that the plant will take in water until it is sufficiently rehydrated and then it will stop. Materials (9A) : ring stand, tubing, pipette, clamp, potometer, plant Procedure (9A) : 1. Place the tip of a 0.1-mL pipette into a 16-in piece of clear plastic tubing. 2. Submerge the tuning and pipette in a shallow tray of water. Draw water through the tubing until all the bubbles are eliminated. 3. Carefully cut the plant stem under the water, being sure to avoid air bubbles. 4. While the plant and tubing are submerged, insert the freshly cut stem into the open end of the tubing. 5. Bend the tubing into a “U” and use the clamp on a ring stand to hold both the pipette and the tubing.

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