Diffusion and Osmosis Lab

Diffusion and Osmosis Lab - Natalie Cook AP Biology Lab 1...

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Natalie Cook               Lab 1  AP Biology    21 September 2007 Title - Diffusion and Osmosis Objectives - To observe the processes of diffusion and osmosis in a model membrane system; to  investigate the effect of solute concentration on water potential as it relates to living plant tissues Hypothesis - I hypothesize that a more noticeable change in color will indicate an increased  presence of glucose, which I predict will increase the mass of the solutions. Materials - distilled water, 250mL beaker, dialysis tubes, glucose solution, .2  sucrose, .4  sucrose, .6  sucrose, .8  M  sucrose, 1.0  sucrose, potato slices Procedure A 1. Obtain pieces of presoaked dialysis tubing, and tie off one end of each to form bags 2. Test the 15% glucose/ 1% starch solution for the presence of glucose 3. Place 15mL of the 15% glucose/ 1% starch solution in the bag. Tie off the free end of the  bag, leaving adequate space for eventual expansion of the bag’s contents.  4. Fill beaker 2/3 fill with distilled water, add about 4mL of Lugol’s solution to the distilled  water and record the color of the water 5. Immerse the bag in the beaker of solution 6. Allow experiment to stand for 30mins and record the final color of the bag’s solution 7. Test the liquid in the beaker and in the bag for the presence of glucose. Data Observations Table Initial Solution Color  Presence of Glucose
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Contents Initial Final Initial Final Bag 15%  glucose/ 1%  starch Colorless,  translucent Dark violet Present Present Beaker H 2 Lemon  yellow Pale Yellow Absent Present Analysis Questions 1. Which substance(s) are entering the bag and which are leaving the bag? What  experimental evidence supports y our answer? Water diffuses into the dialysis bag and glucose leaves. This is indicated by the beaker 
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Diffusion and Osmosis Lab - Natalie Cook AP Biology Lab 1...

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