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Unformatted text preview: LAB 4: Plant Pigments and Photosynthesis Objective: The objective of this lab is to compare the photosynthetic rates at varying light intensities and wavelengths using a controlled experiment. Hypothesis: The photosynthetic rate in plants will be higher in the plants with unboiled chloroplasts in the light. Procedure: 1. Turn on the spectrophotometer to warm up the instrument and set the wavelength to 605 nm. 2. Set up an incubation area that includes a light, water flask, and a test tube rack. 3. Keep the beakers of boiled and unboiled chloroplasts on ice at all times. 4. Label the cuvettes 1 through 5. Using, lens tissue, wipe the outside walls of each cuvette. Cover the walls and bottom of cuvette 2 with foil and make a foil cap to cover the top. 5. To each cuvette, add 1 mL of phosphate buffer. To cuvette 1, add 4 mL of distilled water. To cuvettes 2, 3, and 4, add 3mL of distilled water and 1 mL of distilled water....
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