Defining the mesenchymal stem cell

Defining the mesenchymal stem cell - Defining the...

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efining the mesenchymal stem cell Defining the mesenchymal stem cell Dr. Catherine H. Radcliffe Graduate Student in Comparative Biomedical Sciences Feb 26, 2007
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The mesenchymal stem cell (MSC)
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Mesenchymal terminology • Mesenchymal stem cells – Multipotent omo eneous opulation Homogeneous population – Needs to fulfill strict criteria of “stemness” esenchymal romal ell • Mesenchymal stromal cell – A mixed population of stem cells found in bone marrow or other tissue that does not give rise to blood cells but instead generates +/- bone, cartilage, fat, and fibrous connective tissue eterogeneous Heterogeneous •C l o n e line of genetically identical cells which then give EM Horwitz et al. Cytotherapy (2005) Vol. 7, No. 5, 393/395 – A line of genetically identical cells which then give rise to all differentiated cell types of the tissue http://stemcells.nih.gov/info
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otential sources Potential sources • Bone marrow • Umbilical cord blood (Wharton’s jelly) • Muscle • Fat • Blood eriosteum • Periosteum • Tendons/ligaments •P lacenta •L i v e r • Niche of the MSC???
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efining the MSC Defining the MSC • Cell surface protein characteristics* • Behavior in culture (putative)* – Adherence to plastic plate – Rapid expansion • Induction* – Ability to express mRNA/proteins of “differentiation” nalysis • In vivo analysis – Transplantation ngraftment and function – Engraftment and function *Pittenger et al. Science 1999 Volume 284
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herapeutic applications Therapeutic applications • Tissue engineering one formation (osteogenesis imperfecta fx repair) – Bone formation (osteogenesis imperfecta, fx repair) – Cartilage grafting – Diabetes mellitus ( β pancreatic island cells) – Tendonitis • Graft vs host disease nhibition of dendritic cell differentiation Inhibition of dendritic cell differentiation – Bone marrow transplantation (allografts) – Glomerulonephritis • Heart disease – Antiinflammatory effect – Cellular recruitment Strauer et al., 2002 Chen et al. 2004 Katritsis et al. 2005 • Transdifferentiation??? Giordano A. et al J. Cell. Physiol. 211: 27–35, 2007.
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hallenges to MSC research Challenges to MSC research ack of consistent definition Lack of consistent definition • Lack of standard isolation and culture rotocol protocol • Tissue source variability o successful ssay for • No successful in vivo assay for “stemness” to date o single antibody is specific for MSCs No single antibody is specific for MSCs • Basic research is now emphasized less an therapeutic trials than therapeutic trials www.Clinical.Trials.gov of United States National Institute of Health
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This note was uploaded on 09/28/2008 for the course BIO 4400 taught by Professor Tumbar,td during the Spring '07 term at Cornell University (Engineering School).

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Defining the mesenchymal stem cell - Defining the...

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