This preview shows page 1. Sign up to view the full content.
Unformatted text preview: 1) Mix 56.8 g starch with 250 mL de-ionized water and reduce to a pH of 5.4 using the glacial acetic acid and sodium hydroxide to create a buffer. 2) Heat mixture to between 105-110 C. 3) Add 5 mL of Termamyl Supra -amylase and react for 5 min. 4) Cool to 95 C react for 30 min for 1 sample and 90 min for the second sample. 5) Cool mixture to 60 C, reduce pH to 4.3 and add 5 mL Dextrozyme E and react for the rest of the class period taking fraction every 15 min and placing each fraction in a cool place (i.e. the freezer)....
View Full Document
- Spring '07