Unformatted text preview: 1) Mix 56.8 g starch with 250 mL de-ionized water and reduce to a pH of 5.4 using the glacial acetic acid and sodium hydroxide to create a buffer. 2) Heat mixture to between 105-110 °C. 3) Add 5 mL of Termamyl Supra α-amylase and react for 5 min. 4) Cool to 95 °C react for 30 min for 1 sample and 90 min for the second sample. 5) Cool mixture to 60 °C, reduce pH to 4.3 and add 5 mL Dextrozyme E and react for the rest of the class period taking fraction every 15 min and placing each fraction in a cool place (i.e. the freezer)....
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- Spring '07
- glacial acetic acid, -Glacial Acetic acid