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Unformatted text preview: Extracting and Amplifying mtDNA Amrita Tharappel Partner: Kito Barrow & Maryanne Mani Section 102 April 3, 2006 1. Cathode Anode (-) (+) Kito Mary- Anne Amrita 100 200 300 400 500 600 700 800 900 1000 2. What is the estimated size of your PCR product based on your gel? Does it agree with the predicted size of the product based on the locations of the primers? The estimated PCR product based on the gel is around 550 bp. Yes it agrees with the product based location of the primers 3. Each round of PCR doubles the number of DNA molecules present in the reaction. How many molecules can be generated from one of your DNA template molecules after 30 cycles? 2^30 = 1,073,741,824 molecules 4. You amplified a segment of your own mitochondrial DNA. Do you expect the sequences of your PCR product vary from those of your classmates? Why or why not? Yes the sequences will vary because we inherit our mitochondrial DNA from our mothers and we all have different mothers, so hence the sequences will be different. The gel results of the experiment will have different mothers, so hence the sequences will be different....
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- Spring '08