Report 5 - 4. The major source of error in the patching...

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Yeast Genetics Amrita Tharappel Partner: Kito Barrow Section 102 February 20, 2006
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2. HAO HA1 HA2 HA12 HBO W W W W HB1 W R W R HB2 W W R R HB12 W R R R Yes the color matched our predictions. The cross between the HA2 strain and the HB1 strain formed the cream color mixture even though the HA2 strain and the HB1 strain are red themselves. The mutations that are missing in HA2 is in a different location than the one on HB1 and so the two will show complements of each other. 3. HAO HA1 HA2 HA12 HBO + W + W + W + W HB1 +W - +W - HB2 +W +W - - HB12 +W - - - Yes the growth and the color matched with our predictions once again. The growth on the MV plate of the mix of strain HA1 and HB2 is the same theory behind the growth of the strain HA2 and HB1. The two strains have their missing mutations in different locations and thus will complement each other. If the mutations where in the same location the two strains would be the same type and will not grow on the MV plate, but since they are different, growth can appear.
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Unformatted text preview: 4. The major source of error in the patching technique when red blood cells still appear is us first of all putting too many cells on the MV plate, and then we would not have mixed the two strains together on the YED plate well enough and in transferring the red color transferred as well. 5. When excess adenine is present the ade1 mutant will not form red pigments. If there is an excess of adenine on the growth medium it does not sense a need to synthesize its AMP. So if an excess of adenine exists in the medium the cell will stop producing its AMP and it will prevent other nutrients such as air from providing the cell with nutrients so it can synthesize. Also because the excess of adenine stops the air from entering the cell, the cell can not turn red, the red mutants can not turn red when there is no oxygen available....
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Report 5 - 4. The major source of error in the patching...

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