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Unformatted text preview: 4. The major source of error in the patching technique when red blood cells still appear is us first of all putting too many cells on the MV plate, and then we would not have mixed the two strains together on the YED plate well enough and in transferring the red color transferred as well. 5. When excess adenine is present the ade1 mutant will not form red pigments. If there is an excess of adenine on the growth medium it does not sense a need to synthesize its AMP. So if an excess of adenine exists in the medium the cell will stop producing its AMP and it will prevent other nutrients such as air from providing the cell with nutrients so it can synthesize. Also because the excess of adenine stops the air from entering the cell, the cell can not turn red, the red mutants can not turn red when there is no oxygen available....
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- Spring '08