Midterm_IIs06key

Midterm_IIs06key - LS4 MIDTERM II INSTR: L. JOHNSON NAME:_...

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1 LS4 MIDTERM II INSTR: L. JOHNSON Friday, Sept 1, 2006 NAME:_________________________________ (last) (first) ID #___________________________________ INSTRUCTIONS 1. This exam is 6 pages long. Problem Score Before starting make sure you have all the pages. 1 10 2. No credit will be given for an 2 10 illegible answer. Please write in pen. 3 25 3. You may use a calculator. 4 15 5 15 6 25 Total 100 KEY Name _________________________________________ID#______________________
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2 (last) (first) (10 pts) 1. The following Hfr strains were used in mapping several E. coli genes. Construct a genetic map of these loci, indicating with an arrowhead the location of each of the Hfr strains. Time elapsed before cells carrying markers were recovered Hfr strain 5min 10min 15min 20min 25min 30min 1 pro gal lac 2 pur ala leu lac 3 pro thy thr 4 pur ile thr 5 thy pro gal (10 pts) 2 The lacZ and lacY genes map very close to one another on the bacterial genome. To determine the order of lacZ and lacY with respect to a distal gene proC, a cross was made between Hfr str s lacZ - lacY + proC + and F - str r lacZ + lacY - proC - . After about 2 hours, the mating mixture was diluted and plated out on medium containing streptomycin but no proline. When the resulting
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This note was uploaded on 09/30/2008 for the course LIFESCI 4 taught by Professor Ribaya during the Spring '08 term at UCLA.

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Midterm_IIs06key - LS4 MIDTERM II INSTR: L. JOHNSON NAME:_...

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