MidtermII_f05key

MidtermII_f05key - LS4 Midterm II INSTR: L. Johnson...

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LS4 Midterm II INSTR: L. Johnson Thursday, Nov. 17, 2005 NAME:_________________________________ (last) (first) I.D. #__________________________________ INSTRUCTIONS 1. This exam is 6 pages long. PROBLEM VALUE SCORE Before starting make sure you have all the pages. 1 16 2. Be sure to write clearly. Only 2 14 legible answers will be graded. 3 13 4 22 5 15 6 20 Total 100 KEY
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2 NAME:_________________________________ ID# _______________________ (last) (first) (16 pts) 1. Six Neurospora strains auxotrophic for proline were crossed to each other to determine complementation and linkage relations. First growth of the heterokaryons on minimal media was recorded for each pair (Table 1). Then diploids from each combination were made and allowed to go through meiosis. 500 haploid spores from each cross were germinated on minimal media. The number of spores that grew into colonies is shown in Table 2. Table 1 proA proB proC proD proE proF proA - + + + - + proB - + - + + proC - + + + proD - + + proE - + - Table 2 proA proB proC proD proE proF proA 0 125 37 125 75 proB 0 125 125 62 proC 0 125 113 proD 0 125 62 proE 0 75 proF a) How many complementation groups are found amongst these mutants? Write the complementation groups and which alleles belong to each. A,E B,D C F b) Draw a genetic map consistent with the information provided above. Be sure to indicate mu where appropriate. B,D 24.8 mu F 30 mu A,E 14.8 mu C
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3 NAME:_________________________________ ID# _______________________ (last) (first) (14 pts) 2. In yeast, the intermediates A, B, C, D, E and F all occur in the same biochemical pathway. Eight auxotrophic mutants are isolated which can grow in the presence of various intermediates. A (+) in the table indicates that the strain will grow if given that substance; an (O) means lack of growth.
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MidtermII_f05key - LS4 Midterm II INSTR: L. Johnson...

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